WebDec 31, 2024 · The h ALDH1L1 ALDH1L1 locus was assessed for genomic conservation using the Vistabrowser2 []. h ALDH1L1 promoter was generated into MluI-BamHI restriction enzyme sites of AAV-MCS (multi cloning site) expression vector (Cell Biolabs, Inc. cat# VPK-410) by in-Fusion cloning scheme as described previously []. AAV-hALDH1L1-Cre … WebSTOCK Tg(Aldh1l1-cre)PB1Gsat/Mmucd. Stock Number: 036071-UCD. Citation ID: RRID:MMRRC_036071-UCD Copy RRID Copy Citation. Other Names: STOCK Tg(Aldh1l1-cre)PB1Gsat/Mmcd. Major Collection: GENSAT. Strain Information Expand All. Gene Details [Including genotyping protocols] cre. Name: cre recombinase. Type: Gene ...
ALDH1L1 - Wikipedia
WebThese mice allow pan-astrocytic, specific and inducible genetic manipulations in vivo for studying astrocyte biology at different developmental stages in neural circuitry/synapses, … WebApr 4, 2024 · Mutation details : The transgene was generated by inserting a cre/ERT2 cassette into intron 1/ exon 2 of the mouse Aldh1l1 in a murine BAC (BAC RP23-7M9) by homologous recombination in bacteria. Pronucleus injection gave rise to 5 transgenic founder mice. Three out of five founder mice that were crossed with reporter mice … dr gayle mosher wayland ny
Expression profile of Aldh1L1-GFP labels gliogenic radial glia and ...
WebAldh1l1 Cre displays Cre expression not only in astrocytes but also in some oligodendrocytes during early development (Tien et al., 2012) and Aldh1l1 Cre/ERT2 could potentially cause significant ... WebAldh1l1-Cre/ERT2 x RiboTag mice and the determination of the cortical astrocyte transcriptome at P80 A. Representative photomicrographs of IHC data showing strong colocalisation between S100β and... WebThe genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the ... dr. gayle matthews