NettetWith the addition of Hoechst 33342, a specific DNA fluorochrome, we can correlate apoptotic cell death with cell cycle analysis. Unlike other DNA dyes, Hoechst 33342 … NettetLive Cell Cycle Analysis with Hoechst 33342 1. Harvest cells washing in PBS. 2. Add 10 µg/ml Hoechst 33342 to cells incubate at 37oC for 45 minutes 3. Transfer cells to …
A Triple Staining Method for Accurate Cell Cycle Analysis Using
NettetAfter incubation, the cells were stained with Hoechst 33342 for 10 min and rinsed twice with cold PBS. ... Anticancer drug targets: cell cycle and checkpoint control. J Clin Invest. 1999;104(12):1645–1653. Supplementary materials. Materials and methods. Materials. Nettet18. jun. 2016 · Compound 1 induced G1 phase cell cycle arrest in A2780 cells. Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. introduction to networking and terminology
Effects Of Adenosine On Apoptosis Of Ovarian Cancer A2780 Cells …
http://www.icms.qmul.ac.uk/flowcytometry/flowcytometry/guides/Cell%20Cycle%20Tutorial.pdf Nettet14. des. 2016 · In contrast to previous reports [40, 41], we did not see major effects of Hoechst 33342 on cell cycle duration or significant phototoxicity upon FLIM imaging (S5 and S6 Figs). The latter can be explained by much lower laser powers employed in TCSPC-FLIM method [ 15 ], broadening its applicability to live cell imaging. NettetHoechst 33342 and propidium iodide are frequently used together for simultaneous flow cytometric and fluorescence imaging analysis of the stages of apoptosis and cell-cycle … introduction to networking training