Web1 Dec 2024 · The liver is highly vascularized in its nature, making it the most common site for cancer development, especially metastatic cancers. According to the GLOBOCAN … Web我这里有mda-231、mcf-7、huh7、lm3可以交换 丁香园 - 细胞生物与生物信息 - 2024-09-01 17:21:48 请问，为啥 MCF -7换成无酚红培养基后形态发生改变?
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Web12 Dec 2024 · Two types of HCC cells, Huh7 and LM3 were co-cultured as a model to test whether the method is feasible for cell quantitation. The results indicated that the … Web20 Apr 2024 · LM3, Huh7, Panc02, and RBE cell lines were cultured as organoids in the presence or absence of lactate, and total protein was extracted to measure the …
Web1 Mar 2015 · HCC cell lines (HepG2, Huh7 and LM3), normal hepatic cell line (L-02) and primary rat hepatocytes were grown in 96-well plates for 24 h and subjected to PL treatment (0, 5, 10, 15 or 20 μM) for 24 h. Cell viability was measured by MTT assays (n=3). All values represented mean ± SEM of three independent experiments. Web10 Jan 2024 · The transfected HCC cells (Huh7, LM3, and Hep-G2) were seeded in 96-well plates (1,000 cells/well). Cell proliferation was measured every 24 h for 5 days using the …
WebBoth Huh7 and MHCC-LM3 cells were treated with MG132 (20 μM, 6 h), which is a general proteasome inhibitor. H The ubiquitylated HMGB1 protein was detected by western blot after HMGB1 immunoprecipitation in HCC cells. Cells were treated with MG132 (20 μM, 6 h) or the VCP inhibitor NMS873 (10 μM, 6 h). WebHuh7 and MHCCLM3 cells which were transfected with si-NC and si-SNHG4, respectively, were harvested with RIPA lysis buffer containing 1 mM Na 3 VO 4, 1 mM Cocktail and 1 mM PMSF. Cell debris was removed by centrifugation. The total protein concentration was determined by the BCA protein assay.
WebHepatocellular carcinoma (HCC) is the world's third leading cause of cancer death, with metastasis being the predominant cause of treatment failure. We aim to explore the role of Talin1 in HCC and its underlying mechanism. We conducted bioinformatic analysis, qRT-PCR, Immunohi...
Web18 Sep 2024 · For transfection, Huh7 or LM3 cells were plated into 6-well plates and incubated for 24 h. Then, the cells were transfected with pcDNA3.1-KCNQ1 using … hernan piniellaWeb4 Mar 2024 · The TCF4 expression plasmid pcDNA3.1-TCF4 was generated by insertion of the full-length ORF of TCF4 into the Kpn I and Xho I sites of pcDNA3.1 vector … hernan rosasWeb15 Sep 2024 · Human HCC cell lines, including LM3, Huh7, PLC/PRF/5, and Hep3B were purchased from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences, Shanghai, China). HCC cell lines were incubated in DMEM (Gibco, Gaithersburg) with 10% fetal bovine serum and maintained at 37℃ in a humidified incubator with … hernansosa94Web27 Aug 2024 · Hepatocellular carcinoma (HCC) is one common malignant tumor of the digestive system, and is a major threat to public health, especially in China [ 1 ]. It is a heterogeneous malignant tumor and has the sixth highest morbidity and fourth highest mortality in the world [ 2 ]. hernan orjuela historiaWebThe Huh7, LM3 and Hep3B cell lines have high invasive characteristics, whereas the YY‑8103, SNU‑398 and MHCC‑97L cell lines have less invasive characteristics (18). Cell transfection. A KDM6A coding sequence was constructed and inserted into the p23-3xflag-GFP vector (Invitrogen; Thermo Fisher Scientific, Inc.) to generate a KDM6A overex- hernan silva silvaWeb"J 111;人单核细胞白血病易增殖细胞 配套专用培养基 BS-C01604423 背景信息：单核细胞白血病 产包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支) 尽管无法完全 … hernan possettiWebHepatocellular carcinoma cell lines (Hep3B, SMMC-7721, LM3 and Huh7) and L02 were purchased from the American Type Culture Collection (ATCC, Manassas, VA), and cultured in complete culture medium (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), 100 U/ml penicillin (Gibco), and 100 … hernan raskin