Web1 jan. 2009 · 3 The Human CMV Major Immediate Early Promoter Region The major immediate early (MIE) region from the human cytomegalovirus (CMV) is one of the … WebIn the art there is still a need to enhance the activity of the mCMV promoter. Therefore, the technical problem underlying the present invention is to provide a mCMV promoter …
The activator protein 1 binding motifs within the human …
Web28 apr. 2024 · We therefore deduced that the two Sp1 sites act as an upstream activator element ... To further investigate the impact of proximal enhancer, we truncated the 5’ … Web5 sep. 2024 · CMV, Cytomegalovirus major immediate-early; CAG, the CMV enhancer fused to the chicken beta-actin promoter; CHEF-1α, Chinese hamster elongation factor … first oriental market winter haven menu
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Web12 mei 2010 · To facilitate more rational choices of promoters in ectopic gene expression studies, we decided to examine six constitutive promoters commonly used in … Web14 apr. 2024 · To construct the luciferase gene expression vectors, the phosphoglycerate kinase (PGK) promoter of the pmirGLO plasmid was replaced with the MIEP enhancer and promoter sequence form the plasmid of pHAGE, generating pmirGLO-MIEP pro-luc. 293T cells in 12-well plates (2.5 × 10 5 cells/well) were transfected with pmirGLO-MIEP pro-luc … Web9 mei 2007 · Enhancers are defined as DNA elements that increase transcription when placed in any orientation relative to a promoter. The major immediate-early (MIE) enhancer region of murine cytomegalovirus is flanked by transcription units ie1/3and ie2, which are transcribed in opposite directions. first osage baptist church