NettetThe Immortalized Mouse Astrocytes- SV40 T (IMA 2.1) is a suitable cell line for studies in astrocyte functions. This cell line is unique in that it is capable of metabolizing the parkinsonian toxin MPTP (to MPP+) and is responsive to pro-inflammatory stimuli. In addition, this cell line can be used in neuronal co-cultu Nettet1. jun. 2024 · Highly enriched primary astrocytes were prepared from the cerebral …
Isolation and Culture of Mouse Cortical Astrocytes Protocol - JoVE
Nettet13. mar. 2024 · In vitro analysis using primary mouse astrocytes exposed to T. gondii excreted-secreted antigens (TgESAs) resulted in polarization to an A1-like phenotype, independent of microglia, as evidenced by upregulated reactive astrocyte transcript expression and C3 levels (Fig. 1 j). Nettet13. apr. 2024 · Primary cultured astrocytes were transfected with lentiviruses and then ... Tanonaka K. Effects of Hsp90 inhibitor on the RIP1-RIP3-MLKL pathway during the development of heart failure in mice. massive action llc
Characterization of mouse cell line IMA 2.1 as a potential
NettetHypoxic ischemic injury to the fetal and neonatal brain is a leading cause of death and disability worldwide. Although animal and culture studies suggest that glutamate excitotoxicity is a primary contributor to neuronal death following hypoxia, the molecular mechanisms, and roles of various neural cells in the development of glutamate … Nettet19. des. 2007 · Staining & Characterization. Incubate cells in 5% goat serum diluted in D … NettetWe have demonstrated the feasibility of this approach on the example of WT- and 3Tg-iAstro cells generated from primary hippocampal astrocytes of 3xTg-AD mice. In several reports and inter-lab collaborations, we confirmed that 3Tg-iAstro cells maintain many physio-pathological features of AD astrocytes while being extremely versatile in terms … hydroseal ball valve maintenance manual