The sybr premix ex taq kit
WebAug 17, 2016 · The PCR amplification was performed on MasterCycler RealPlex 4 (Eppendorf, USA) using SYBRTM Premix Ex TaqTM II Kit (Takara) according to the manufacturer’s instructions. The reaction mixture in a 20 μL volume contained 2 μL (100 ng) of cDNA, 1.6 μL (0.4 μM) mixture of the forward and reverse primers (sequences listed in … Web报价. SYBR Premix EX Taq (Perfect Real time染料法实时荧光定量试剂盒) DRR041A. Takara. 200次. ¥2,000. 促销期2012年3月1日至5月30日。. TAKARA-Real Time PCR 试剂使 …
The sybr premix ex taq kit
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Web36181981 supply of faststar sybr green master , faststart sybr green master, 5 ml ( 10 × 5 ml ) kit should contain faststart taq dna polymerase for hot start pcr ready to use, 2× concentrated master mix that contains all the reagents ( except primers and template ) needed for running quantitative, real time dna detection assays, including ... WebNational Center for Biotechnology Information
WebFeb 25, 2024 · The DNeasy ® PowerSoil ® Kit (Qiagen) ... The 20 μL qPCR mixture for quantifying nirS and nosZ genes contained 10 μL SYBR Premix Ex Taq, 0.25 μL (50 μmol/L) forward primer, 0.25 μL (50 μmol/L) reverse primer, 1 μL tenfold diluted DNA template, and 8.5 μL sterile water. WebFinally, RT-qPCR was carried out for 40 cycles (95°C for 5 seconds and 60°C for 34 seconds) using SYBR™ Premix Ex Taq kit on an 7500 RT-PCR Instrument (Thermo Fisher Scientific). The relative expression of p53 gene was calculated and normalized by 2 −ΔΔCT method, using β-actin as a reference gene. The primers were used as follows:
WebJan 8, 2007 · The Ex Taq™ HS is an enzyme for hot start PCR which utilizes an anti-Taq antibody. The SYBR® Premix Ex Taq™ mix also contains dNTPs, Mg2+ and a newly … Web本制品对 SYBR Green I 浓度、PCR 反应体系都进行了优化，采用了反应性能优越的 Pro Taq HS DNA polymerase 体系，能够有效抑制非特异性产物的扩增，提高 PCR 扩增效率，可以进行高灵敏度的 Real Time PCR 反应，在宽广的定量区域内得到良好的标准曲线，从而对靶基因 …
WebMar 9, 2024 · RNA was extracted and reverse transcribed using All-In-One RT MasterMix (ABM). qRT-PCR was performed using SYBR Premix Ex Taq (Takara). The transcript abundance of candidate genes was calculated using the 2− Ct method (Livak & Schmittgen, 2001). The actin gene was amplified as a reference.
Web• SYBR Green Taq ReadyMix for Quantitative RT-PCR, Catalog No. D5191, 2× 50 RXN 20 mM Tris-HCl, pH 8.3, 100 mM KCl, 6 mM MgCl 2, 0.4 mM each dNTP (dATP, dCTP, dGTP, TTP), stabilizers and glass passivator, 1 U/µL RNase Inhibitor, Taq DNA Polymerase, JumpStart Taq antibody, and SYBR Green I dye. • M-MLV Reverse Transcriptase, Catalog Number metlife roth iraWebWang et al. developed a clinical-grade bioartificial liver device with cryopreserved GMP-compliant human-induced hepatocytes (hiHep-BAL). hiHep-BAL treatment improved functions and regeneration in pigs with post-hepatectomy liver failure and increased their survival. Moreover, a clinical study demonstrated the feasibility and safety of hiHep-BAL in … how to add spotify widget on notionWebApr 10, 2024 · RT reagent Kit with gDNA Eraser (TaKaRa). For the nested PCR, the primers targeted on the N gene of BJNV were designed based on the results of viral metagenomics (Table S1). The 25?L first-round PCR mixture included 2.5?L 10 PCR reaction buffer, 50?mM MgCl2, 5?pmol of each primer, 1?L cDNA 0.5?mM dNTP, and 0.1?L ExTaq Enzyme (Takara). metlife salary grade scaleWebDownload scientific diagram GPNA treatment inhibited MDA-MB-231 and BT-549 cell processes. (a-c) SLC1A5 expression at mRNA and protein level was detected by qRT-PCR and Western blot analysis ... metlife rhode island warwickWeb本制品对 SYBR Green I 浓度、PCR 反应体系都进行了优化，采用了反应性能优越的 Pro Taq HS DNA polymerase 体系，能够有效抑制非特异性产物的扩增，提高 PCR 扩增效率，可以 … how to add spotify widget on windows 11metlife r\\u0026s ace pension lexington kyWebRT-qPCR was performed using the SYBR Premix Ex Taq II (TaKaRa, DRR081A) and conducted using a Stratagene Mx3000 real-time system (USA). A reaction volume of 20 µL with 1 µL forwarding primer (0.4 mmol each), 1 µL reverse primer (0.4 mmol each), 10 µL 2× Es Taq MasterMix, and 6 µL RNasE-free ddH 2 O was used. how to add spouse to property deed in texas